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Sequential Gene Silencing Using Wavelength‐Selective Caged Morpholino Oligonucleotides |
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| Authors: | Dr Sayumi Yamazoe Qingyang Liu Dr Lindsey E McQuade Prof?Dr Alexander Deiters Prof?Dr James K Chen |
| Institution: | 1. Departments of Chemical and Systems Biology and Developmental Biology, Stanford University School of Medicine, 269 Campus Drive, CCSR 3155, Stanford, CA 94305 (USA) http://chen.stanford.edu;2. Department of Chemistry, University of Pittsburgh, Chevron Science Center, 219 Parkman Avenue, Pittsburgh, PA 15260 (USA) http://www.pitt.edu/～deiters |
| Abstract: | Spectrally differentiated caged morpholino oligonucleotides (cMOs) and wavelength‐selective illumination have been used to sequentially inactivate organismal gene function. The efficacy of these reverse‐genetic chemical probes has been demonstrated in zebrafish embryos, and these reagents have been employed to examine the mechanisms of mesoderm patterning. |
| Keywords: | antisense agents cage compounds developmental biology gene expression oligonucleotides |