Imaging Lipid Metabolism in Live Caenorhabditis elegans Using Fingerprint Vibrations |
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| Authors: | Dr. Ping Wang Bin Liu Delong Zhang Micah Y. Belew Prof. Heidi A. Tissenbaum Prof. Ji‐Xin Cheng |
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| Affiliation: | 1. Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907 (USA);2. Department of Chemistry, Purdue University, West Lafayette, IN 47906 (USA);3. Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605 (USA) |
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| Abstract: | Quantitation of lipid storage, unsaturation, and oxidation in live C. elegans has been a long‐standing obstacle. The combination of hyperspectral stimulated Raman scattering imaging and multivariate analysis in the fingerprint vibration region represents a platform that allows the quantitative mapping of fat distribution, degree of fat unsaturation, lipid oxidation, and cholesterol storage in vivo in the whole worm. Our results reveal for the first time that lysosome‐related organelles in intestinal cells are sites for storage of cholesterol in C. elegans. |
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| Keywords: | C. elegans cell metabolism lipids stimulated Raman scattering vibrational spectroscopy |
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