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Bioorthogonal Cleavage and Exchange of Major Histocompatibility Complex Ligands by Employing Azobenzene‐Containing Peptides |
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| Authors: | Joanna A. L. Choo Dr. Sock Yue Thong Jiawei Yap Dr. Wim J. E. van Esch Dr. Manfred Raida Dr. Rob Meijers Dr. Julien Lescar Dr. Steven H. L. Verhelst Dr. Gijsbert M. Grotenbreg |
| Affiliation: | 1. Department of Microbiology and Immunology Programme, National University of Singapore (NUS), Life Sciences Institute, #03‐05, 28 Medical Drive, 117456 (Singapore);2. Division of Reagents, Sanquin Blood Supply, 1066 CX Amsterdam (The Netherlands);3. SLING, National University of Singapore (NUS), Life Sciences Institute, #03‐03, 28 Medical Drive, 117456 (Singapore);4. European Molecular Biology Laboratories, Hamburg Outstation c/o DESY, Notkestra?e 85, 22603 Hamburg (Germany);5. School of Biological Sciences, Nanyang Technological University (NTU), 60 Nanyang Drive, 637551 (Singapore);6. Center for Integrated Protein Science Munich, Lehrstuhl Chemie der Biopolymere, Technische Universit?t München, Weihenstephanerberg 3, 85354 Freising (Germany);7. Department of Biological Sciences, National University of Singapore (NUS), 14 Science Drive 4, 117543 (Singapore) |
| Abstract: | Bioorthogonal cleavable linkers are attractive building blocks for compounds that can be manipulated to study biological and cellular processes. Sodium dithionite sensitive azobenzene‐containing (Abc) peptides were applied for the temporary stabilization of recombinant MHC complexes, which can then be employed to generate libraries of MHC tetramers after exchange with a novel epitope. This technology represents an important tool for high‐throughput studies of disease‐specific T cell responses. |
| Keywords: | bioorthogonal chemistry cleavable linkers immunology major histocompatibility complex peptidomimetics |