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Reversible Ca2+ Switch of An Engineered Allosteric Antioxidant Selenoenzyme |
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| Authors: | Chunqiu Zhang Tiezheng Pan Prof Christian Salesse Dongmei Zhang Lu Miao Liang Wang Yuzhou Gao Jiayun Xu Prof Zeyuan Dong Prof Quan Luo Prof Junqiu Liu |
| Institution: | 1. State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, 2699 Qianjin Road, Changchun 130012 ( P.R. China);2. CUO‐Recherche, Centre de Recherche du CHU de Québec, Département d'Ophtalmologie, Faculté de Médecine, and PROTEO, Université Laval, 1050 Chemin Ste‐Foy, QC, G1S4?L8 (Canada) |
| Abstract: | A Ca2+‐responsive artificial selenoenzyme was constructed by computational design and engineering of recoverin with the active center of glutathione peroxidase (GPx). By combining the recognition capacity for the glutathione (GSH) substrate and the steric orientation of the catalytic selenium moiety, the engineered selenium‐containing recoverin exhibits high GPx activity for the catalyzed reduction of H2O2 by glutathione (GSH). Moreover, the engineered selenoenzyme can be switched on/off by Ca2+‐induced allosterism of the protein recoverin. This artificial selenoenzyme also displays excellent antioxidant ability when it was evaluated using a mitochondrial oxidative damage model, showing great potential for controlled catalysis in biomedical applications. |
| Keywords: | antioxidants artificial glutathione peroxidase cysteine‐auxotrophic system recoverin selenocysteine |