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Differential Sensing of MAP Kinases Using SOX‐Peptides |
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| Authors: | Dr. Diana Zamora‐Olivares Dr. Tamer S. Kaoud Dr. Jiney Jose Prof. Andrew Ellington Prof. Kevin N. Dalby Prof. Eric V. Anslyn |
| Affiliation: | 1. Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station A1590, Austin, Texas 78712 (USA);2. Division of Medicinal Chemistry, The University of Texas at Austin, 1 University Station A1590, Austin, Texas 78712 (USA);3. Faculty of Medical and Health Sciences, University of Auckland, Auckland, 92019 (New Zealand);4. Institute for Cell and Molecular Biology, The University of Texas at Austin |
| Abstract: | Five SOX peptides are used to classify the MAPK groups and isoforms thereof using chemometrics. The score plots show excellent classification and accuracy, while support vector machine analysis leads to the quantification of ERK and an ERK inhibitor concentration in kinase mixtures. Examination of the loading plots reveals cross‐reactivity among the peptides, and some unexpected surprises. |
| Keywords: | biosensors chemometrics differential sensing fluorescent probes mitogen‐activated protein kinases |