| 重金属镉离子人工合成抗原的荧光特性分析 |
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| 引用本文： | 郭明,庞铄权,张璐颖.重金属镉离子人工合成抗原的荧光特性分析[J].分析化学,2009,37(7). |
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| 作者姓名： | 郭明 庞铄权 张璐颖 |
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| 作者单位： | 浙江林学院理学院化学系,临安,311300 |
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| 基金项目： | 国家自然科学基金，浙江省科技厅项目 |
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| 摘 要： | 以1-(4-异硫氰苄基)乙烯基二胺-N,N,N′,N′-四乙酸为双功能螯合剂,偶联Cd2+与血清蛋白质分子, 合成了Cd2+人工抗原.利用荧光光谱分析了Cd2+人工合成抗原的荧光特性并得到合成反应的偶联比为11∶ 1～16∶ 1;通过荧光相图分析表明, Cd2+人工抗原合成过程中血清蛋白质的折叠状态符合"二态模型";偏振荧光光谱检测结果显示, Cd2+人工抗原合成中血清蛋白质色氨酸残基的微区构象发生一定变化,导致载体蛋白质构象改变.
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| 关 键 词： | 镉 人工抗原 荧光分光光度法 |
Detection of Artifical Antigen for Cd by Fluorescence Spectrometry |
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| GUO Ming,PANG Shuo-Quan,ZHANG Lu-Ying.Detection of Artifical Antigen for Cd by Fluorescence Spectrometry[J].Chinese Journal of Analytical Chemistry,2009,37(7). |
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| Authors: | GUO Ming PANG Shuo-Quan ZHANG Lu-Ying |
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| Institution: | GUO Ming,PANG Shuo-Quan,ZHANG Lu-Ying(Department of Chemistry,Zhejiang Forestry University,Lin'an 311300) |
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| Abstract: | Artificial antigen Cd-IEDTA-BSA (HSA) was successfully synthesized by the bifunctional chelating agent IEDTA (isothiocyanobenzylethyl enediamine tetraacetic acid) coupling with cadmium and protein carrier. The fluorescence characteristics of antigen were determined by fluorescence spectrophotometry and the coupling ratio 11∶ 1-16∶ 1 was obtained for the coupling reaction. The folding status of serum albumin during the synthesis process of artificial antigen Cd-IEDTA-BSA (HSA) was analyzed using "fluorescence phase diagram", and the result shows that it was conformed to the "all-or-none" pattern. The polarization fluorescence spectra show that the microenvironments of tryptophan residues in carrier protein have some changes to cause changes in protein conformation. |
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| Keywords: | Cadmium artificial antigen fluorescence spectrophotometry |
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