表面增强拉曼法快速检测猪肉中齐帕特罗残留

齐帕特罗是一种β₂-受体激动剂，也就是俗称的“瘦肉精”,经常被不法商家用于牲畜养殖，目前也没有相关国家标准对其残留限量作出规定。该兽药进入牲畜体内以后可以改变某些营养素的代谢方式，促进牲畜肌肉的生长，对牲畜体内的脂肪进行快速的消耗和转化，从而提高牲畜的瘦肉率。目前对该药物的检测方法主要是液相色谱串联质谱法，该方法具有成本高昂、操作繁琐、耗时较长等缺点，表面增强拉曼光谱法具有灵敏度高、检测速度快等优势，近年来被广泛应用于食品中有毒有害物的检测。为了实现猪肉中齐帕特罗的快速检测，建立了一种猪肉中齐帕特罗残留的表面增强拉曼光谱快速检测方法。通过优化比较一系列实验结果，确定样品与金胶的最佳体积比为1∶2以及最优混匀检测时间为3 min;通过对多种萃取溶剂的比较最终确定使用乙酸乙酯为提取剂；通过密度泛函理论中B3LYP/6-311+G(d)基组对齐帕特罗的理论光谱进行计算，确定齐帕特罗的SERS特征峰并进行振动归属，以1 116, 1 447和1 573 cm^-1处的特征峰作为齐帕特罗的定量特征峰，其中1 116 cm^-1是苯环面...

Papid Detection of Zilpaterol Residues in Pork by Surface-Enhanced Raman Spectroscopy

Zilpaterol is a β₂-receptor agonist, also known as “Clenbuterol”, often used in livestock breeding by illegal businesses. There is no relevant national standard that stipulates its residue limit. After the veterinary drug enters the animal’s body, it can change the metabolic mode of certain nutrients, promote the growth of the animal’s muscle, and rapidly consume and transform the fat in the animal’s body, thereby increasing the lean meat rate of the animal. The current detection method for the drug is mainly liquid chromatography-tandem mass spectrometry, which has the disadvantages of high cost, cumbersome operation, and long time-consuming. Surface-enhanced Raman spectroscopy has the advantages of high sensitivity and fast detection speed. In recent years, it has beenwidely used to detect toxic and harmful substances in food. In order to realize the rapid detection of zilpaterol in pork, a surface-enhanced Raman spectroscopy method for rapid detection of zilpaterol residue in pork was established. By optimising and comparing a series of experimental results, it is determined that the best volume ratio of sample to gold glue is 1∶2, and the best mixing detection time is 3 min.Through the comparison of multiple extraction solvents, it is finally determined that ethyl acetate is used as the extractant;through the calculation of the theoretical spectrum of B3LYP/6-311 + G(d) basis group aligned patro in density functional theory, the SERS characteristic peak of zipatro is determined and the vibration is assigned. The characteristic peaks at 1 116, 1 447 and 1 573 cm-1 are taken as the characteristic quantitative peaks of zilpaterol, of which 1 116 cm-1 is caused by the in-plane deformation vibration of a benzene ring, and 1 447 cm-1 is the out of plane swing vibration of C—H bond, 1 573 cm-1 is the stretching vibration of C—H bond of the benzene ring. Under the best experimental conditions, a standard curve of the characteristic peak SERS signal and concentration logarithm of zilpaterol standard solution was established, and the R2 value of the linear equation was all above 0.9; actual samples with different spiked concentrations were performed. The detection showed that the average recovery rate was 80.39%~101.24%, and the RSD value was 7.90%~8.94%. This method is convenient, fast, and stable. It can realize the rapid and accurate determination of zilpaterol residues in pork without complicated sample pretreatment. It provides a new method for detecting zilpaterol and the formulation of related standards.