| STUDY ON INDUCTION OF DIFFERENTIATION OF A HUMAN MEGAKARYOBLASTIC LEUKEMIA CELL LINE (HIMeg) BY 1,25-DIHYDROXYVITAMIN D_3 |
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| 作者姓名： | 程涛 严舫 万景华 殷建林 张淑靖 齐淑玲 宋玉华 |
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| 作者单位： | Department of Hematology，Changhai Hospital，Second Military Medical University，Shanghai 200433，PRC，Department of Hematology，Changhai Hospital，Second Military Medical University，Shanghai 200433，PRC，Institute of Hematology，Chinese Academy of Medical Sciences，Tianjin 300020，PRC，Institute of Hematology，Chinese Academy of Medical Sciences，Tianjin 300020，PRC，Institute of Hematology，Chinese Academy of Medical Sciences，Tianjin 300020，PRC，Institute of Hematology，Chinese Academy of Medical Sciences，Tianjin 300020，PRC，Institute of Hematology，Chinese Academy of Medical Sciences，Tianjin 300020，PRC |
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| 摘 要： | It is reported that 1,25-dihydroxyvitamin D_3(1,25(OH)_2D_3), a physiological factor, has aninductive effect on the differentiation of a novel human megakaryoblastic leukemia cell line(HIMeg) in vitro. At the concentrations ranging from 10~(-9) to 10~(-6) mol/L, 1,25(OH)_2D_3 showedinhibition of proliferation on HIMeg cells which was demonstrated by count of survivalcells and cloning efficiency. Meanwhile, using light/electron microscopy, stain of cytochem-istry (including immunoenzymatic technique) and flow cytometry, we found that HIMeg cellscould be further induced into more mature cells in megakaryocytic lineage confirmed by aseries of evidence, including the changes of cell morphology/structure and cytochemistry,increased expression of differentiation antigens on the cell surface, and polyploidization.So, it is possible for 1,25(OH)_2D_3 to promote the differentiation of the cells in megakaryo-cytic lineage in vivo and to be used to treat acute megakaryoblastic leukemia and other di-seases with mal
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STUDY ON INDUCTION OF DIFFERENTIATION OF A HUMAN MEGAKARYOBLASTIC LEUKEMIA CELL LINE (HIMeg) BY 1,25-DIHYDROXYVITAMIN D_3 |
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| CHENG TAO,YAN FANG,WAN JING-HUA,YIN JIAN-LIN,ZHANG SHU-JING,QI SHU-LING,SONG YU-HUA.STUDY ON INDUCTION OF DIFFERENTIATION OF A HUMAN MEGAKARYOBLASTIC LEUKEMIA CELL LINE (HIMeg) BY 1,25-DIHYDROXYVITAMIN D_3[J].Science in China(Chemistry),1991(4). |
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| Authors: | CHENG TAO YAN FANG WAN JING-HUA YIN JIAN-LIN ZHANG SHU-JING QI SHU-LING SONG YU-HUA |
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| Institution: | CHENG TAO,YAN FANG,WAN JING-HUA,YIN JIAN-LIN,ZHANG SHU-JING,QI SHU-LING,SONG YU-HUA Department of Hematology,Changhai Hospital,Second Military Medical University,Shanghai 200433,PRC Institute of Hematology,Chinese Academy of Medical Sciences,Tianjin 300020,PRC |
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| Abstract: | It is reported that 1,25-dihydroxyvitamin D_3(1,25(OH)_2D_3), a physiological factor, has an
inductive effect on the differentiation of a novel human megakaryoblastic leukemia cell line
(HIMeg) in vitro. At the concentrations ranging from 10~(-9) to 10~(-6) mol/L, 1,25(OH)_2D_3 showed
inhibition of proliferation on HIMeg cells which was demonstrated by count of survival
cells and cloning efficiency. Meanwhile, using light/electron microscopy, stain of cytochem-
istry (including immunoenzymatic technique) and flow cytometry, we found that HIMeg cells
could be further induced into more mature cells in megakaryocytic lineage confirmed by a
series of evidence, including the changes of cell morphology/structure and cytochemistry,
increased expression of differentiation antigens on the cell surface, and polyploidization.
So, it is possible for 1,25(OH)_2D_3 to promote the differentiation of the cells in megakaryo-
cytic lineage in vivo and to be used to treat acute megakaryoblastic leukemia and other di-
seases with malignant megakaryocytosis. |
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| Keywords: | 1 25-dihydr oxyvitamin D_3 HIMeg cells differentiation megakaryocytic lineage leukemia |
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