|
|||||
|
|
Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides |
|
| Authors: | Aya Mizutani Kenichi Nagase Akihiko Kikuchi Hideko Kanazawa Yoshikatsu Akiyama Jun Kobayashi Masahiko Annaka Teruo Okano |
| Institution: | 1. Graduate School of Pharmaceutical Sciences, Keio University, 1-5-14 Shibakoen, Minato, Tokyo 105-0011, Japan;2. Institute of Advanced Biomedical Engineering and Science, Tokyo Women''s Medical University, TWIns, 8-1 Kawadacho, Shinjuku, Tokyo 162-8666, Japan;3. Department of Materials Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan;4. Department of Chemistry, Kyushu University, 6-10-1 Hakozaki, Higashi, Fukuoka 812-8581, Japan |
| Abstract: | Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) P(IPAAm-co-tBAAm)] brushes were prepared on poly(hydroxy methacrylate) (PHMA) hydrolyzed poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)] beads having large pores by surface-initiated atom transfer radical polymerization (ATRP) and applied to the stationary phases of thermo-responsive chromatography. Optimized amount of copolymer brushes grafted PHMA beads were able to separate peptides and proteins with narrow peaks and a high resolution. The beads were found to have a specific surface area of 43.0 m2/g by nitrogen gas adsorption method. Copolymer brush of P(IPAAm-co-tBAAm) grafted PHMA beads improved the stationary phase of thermo-responsive chromatography for the all-aqueous separation of peptides and proteins. |
| Keywords: | Thermo-responsive polymer Poly(N-isopropylacrylamide) Atom transfer radical polymerization Polymer brush Thermo-responsive chromatography |
| 本文献已被 ScienceDirect 等数据库收录！ | |