Adsorption equilibria of proline in hydrophilic interaction chromatography |
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Authors: | Péter Vajda Attila Felinger Alberto Cavazzini |
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Institution: | 1. Department of Analytical and Environmental Chemistry, University of Pécs, Ifjúság útja 6, H–7624 Pécs, Hungary;2. Department of Chemistry, University of Ferrara, via L. Borsari 46, I-44100 Ferrara, Italy |
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Abstract: | The adsorption behavior of proline under hydrophilic interaction chromatography conditions was investigated from six aqueous solutions of acetonitrile. Proline adsorption isotherms were recorded at each mobile phase composition by frontal analysis and inverse method. The BET model was found to be the best choice to describe the nonlinear behavior of proline adsorption under hydrophilic interaction chromatography conditions. The adsorption isotherm parameters were derived from two independent parameter estimation methods. The parameters derived from regression analysis of the frontal analysis data and from overloaded elution bands were found to be in good agreement with the excess isotherm of water. The mobile phase composition at which the maximum excess adsorption of water was observed corresponded to the maximum saturation capacity measured for proline. |
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Keywords: | Proline Frontal analysis HILIC Equilibrium dispersive model |
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