大孔吸附树脂对肿瘤坏死因子吸附性能的研究

选用NK-110、碳化树脂和MET-10043种大孔吸附树脂,通过对树脂吸附量的测定,吸附动力学曲线和吸附等温线的描述等方法,研究了3种大孔吸附树脂对血浆中TNFα的吸附性能,结果表明NK-110和MET-1004对TNFα的吸附量较高,其中又以MET-1004的吸附速率最快

Studies on Adsorption of TNF-α with Macroporous Resin

There are increasing evidences that high serum levels of Tumor Necrosis Factor(TNF-α) have been shown to be associated with disease severity in fulminant hepatic failure and dyscrasia, which is a principal mediator in the cascade of pathophysioligic events following endotoxic shock. In the present study, NK₁₁₀, carbonized resin and MET1004 are selected as adsorbents in the static adsorption experiments of TNF-α. We have investigated the adsorption capacity, kinetic profiles and adsorption isotherm of the three macroporous resins. Experimental results show that_mET1004 and NK₁₁₀ have higher adsorption capacities than carbonized resin. Compared with NK₁₁₀, the velocity of adsorption of MET1004 towards TNF-α is much faster than the former. From experimental data the adsorption rate constants k are calculated to be 0.0163 min^-1 (NK₁₁₀); 0.0189 min^-1 (carbonized resin); 0.0079 min^-1 (MET1004) at 37 ℃, respectively. Furthermore, their adsorption isotherms are also in-different shapes. They are shown to be'H"(MET1004);'L"(NK₁₁₀);'S"(carbonized resin) at 37 ℃, respectively. This demonstrated that the surface to volume ratio and the pore size of macroporous resins had an strong impact on the adsorption capacity and as the pore size increased, the adsorptive rate increased.