| Kinetics of Inactivation of Aminoacylase I During Modification of Its Thiol Groups by DPDS and PCMB |
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| 引用本文： | 王洪睿,阴勇,张英侠,王希成,周海梦. Kinetics of Inactivation of Aminoacylase I During Modification of Its Thiol Groups by DPDS and PCMB[J]. 中国科学B辑(英文版), 1993, 0(1) |
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| 作者姓名： | 王洪睿 阴勇 张英侠 王希成 周海梦 |
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| 作者单位： | Department of Biological Science and Biotechnology，Tsinghua University，Beijing 100084，PRC，National Laboratory of Biomacromolecules，Institute of Biophysics，Academia Sinica，Beijing 100080，PRC)，Department of Biological Science and Biotechnology，Tsinghua University，Beijing 100084，PRC，National Laboratory of Biomacromolecules，Institute of Biophysics，Academia Sinica，Beijing 100080，PRC)，Department of Biological Science and Biotechnology，Tsinghua University，Beijing 100084，PRC，National Laboratory of Biomacromolecules，Institute of Biophysics，Academia Sinica，Beijing 100080，PRC)，Department of Biological Science and Biotechnology，Tsinghua University，Beijing 100084，PRC，National Laboratory of Biomacromolecules，Institute of Biophysics，Academia Sinica，Beijing 100080，PRC)，Department of Biological Science and Biotechnology，Tsinghua University，Beijing 100084，PRC，National Laboratory of Biomacromolecules，Institute of Biophysics，Academia Sinica，Beijing 100080，PRC) |
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| 摘 要： | The kinetics theory of the substrate reaction during modification of enzyme activity previously described by Tsou has been applied to a study on the kinetics of the course of inactivation of aminoacylase I by DPDS and PCMB.From the results obtained we have found that the inactivation reaction of aminoacylase I by DPDS is noncomplexing inhibition,and PCMB reaction is complexing inhibition.The microscopic constants for the reaction of the inactivator with free enzyme and the enzyme-substrate complex were determined.
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Kinetics of Inactivation of Aminoacylase I During Modification of Its Thiol Groups by DPDS and PCMB |
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| WANG Hong-Rui YIN Yong ZHANG Ying-Xia WANG Xi-Cheng and ZHOU Hai-Meng. Kinetics of Inactivation of Aminoacylase I During Modification of Its Thiol Groups by DPDS and PCMB[J]. Science in China(Chemistry), 1993, 0(1) |
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| Authors: | WANG Hong-Rui YIN Yong ZHANG Ying-Xia WANG Xi-Cheng and ZHOU Hai-Meng |
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| Abstract: | The kinetics theory of the substrate reaction during modification of enzyme activity previously described by Tsou has been applied to a study on the kinetics of the course of inactivation of aminoacylase I by DPDS and PCMB.From the results obtained we have found that the inactivation reaction of aminoacylase I by DPDS is noncomplexing inhibition,and PCMB reaction is complexing inhibition.The microscopic constants for the reaction of the inactivator with free enzyme and the enzyme-substrate complex were determined. |
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| Keywords: | aminoacylase chemical modification inhibition kinetics. |
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