| 二乙氨乙基交联葡聚糖和季氨基琼脂糖色谱分离植物脂多糖的比较 |
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| 引用本文： | 陈正行,姚惠源.二乙氨乙基交联葡聚糖和季氨基琼脂糖色谱分离植物脂多糖的比较[J].分析化学,2003,31(7):846-849. |
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| 作者姓名： | 陈正行 姚惠源 |
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| 作者单位： | 江南大学食品学院,无锡,214036 |
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| 基金项目： | 国家科技攻关计划资助项目 (No .2 0 0 1BA5 0 1A0 3 ) |
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| 摘 要： | 比较了应用Q Sepharose Fast Flow凝胶和DEAE-Sephadex A50凝胶柱色谱分离米糠提取物中米糠脂多糖(LPSR)的效果。结果表明：通过改变体系的离子强度，两凝胶都可使LPSR与一般多糖分离，但仅Q Sepharose Fast Flow柱色谱可实现LPSR与米糠色素的有效分离，并获得淡黄色、99．5％纯度的LPSR产品，而且耐盐性能好于DEAE-Sephadex A50。
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| 关 键 词： | 二乙氨乙基交联葡聚糖 季氨基琼脂糖 色谱分离 植物脂多糖 比较 离子交换色诺 米糖 |
Comparison between DEAE-Sephadex and Q Sepharose in Chromatography of Lipopolysaccharide from Plant Origin |
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| Chen Zhengxing,Yao Huiyuan.Comparison between DEAE-Sephadex and Q Sepharose in Chromatography of Lipopolysaccharide from Plant Origin[J].Chinese Journal of Analytical Chemistry,2003,31(7):846-849. |
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| Authors: | Chen Zhengxing Yao Huiyuan |
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| Institution: | Chen Zhengxing*,Yao Huiyuan |
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| Abstract: | The separation of lipopolysaccharides (LRS) in rice bran extract was studied by the column chromatography-DEAE-Sephadex A50 and Q Sepharose Fast Flow, respectively. The results show that by changing ion strength of the system, DEAE-Sephadex A50 can separate lipopolysaccharides from general polysaccharides in rice bran extract while Q Sepharose Fast Flow can effectively separate LPS R from both general polysaccharide and pigment in rice bran extract resulting to fine LPS R product which is pale yellow and has a purity of 99.5%. The performances of Q Sepharose Fast Flow were better than DEAE-Sephadex A50 in separating efficiency and salt-resistance. |
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| Keywords: | Lipopolysaccharide from plant origin rice bran ion exchange chromatography |
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