The SPR sensor detecting cytosine[bond]cytosine mismatches |
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Authors: | Kobori Akio Horie Souta Suda Hitoshi Saito Isao Nakatani Kazuhiko |
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Institution: | PRESTO, Japan Science and Technology Agency (JST), Kyoto 615-8510, Japan. |
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Abstract: | We have synthesized the first surface plasmon resonance (SPR) sensor that detects cytosine-cytosine (Cbond]C) mismatches in duplex DNA by immobilizing aminonaphthyridine dimer on the gold surface. The ligand consisting of two 2-aminonaphthyridine chromophores and an alkyl linker connecting them strongly stabilized the Cbond]C mismatches regardless of the flanking sequences. The fully matched duplexes were not stabilized at all under the same conditions. The Cbond]T, Cbond]A, and Tbond]T mismatches were also stabilized with a reduced efficiency. SPR analyses of mismatch-containing 27-mer duplexes were performed with the sensor surface on which the aminonaphthyridine dimer was immobilized. The response for the Cbond]C mismatch in 5'-GCC-3'/3'-CCG-5' was about 83 times stronger than that obtained for the fully matched duplex. The sensor successfully detects the Cbond]C mismatch at the concentration of 10 nM. SPR responses are proportional to the concentration of the Cbond]C mismatch in a range up to 200 nM. Aminonaphthyridine dimer could bind strongly to the Cbond]C mismatches having 10 possible flanking sequences with association constants in the order of 10(6) M(-1). The facile protonation of 2-aminonaphthyridine chromophore at pH 7 producing the hydrogen-bonding surface complementary to that of cytosine was most likely due to the remarkably high selectivity of 1 to the Cbond]C mismatch. |
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