| 稀土离子(La3+, Gd3+, Yb3+)对线粒体产生活性氧的影响 |
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| 引用本文： | 刘会雪,杨晓达,王夔. 稀土离子(La3+, Gd3+, Yb3+)对线粒体产生活性氧的影响[J]. 高等学校化学学报, 2006, 27(6): 999-1002 |
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| 作者姓名： | 刘会雪 杨晓达 王夔 |
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| 作者单位： | 北京大学药学院化学生物学系,北京,100083 |
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| 基金项目： | 国家自然科学基金(批准号:20101001,20331020)资助 |
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| 摘 要： | 研究了稀土离子对分离的线粒体产生活性氧(ROS)的影响. 采用荧光光度法跟踪线粒体内H2O2生成的动力学, 发现三种稀土离子(La3+, Gd3+, Yb3+)均能降低线粒体H2O2的生成; 用黄嘌呤-黄嘌呤氧化酶体系进一步证明稀土对超氧阴离子(·O-2)存在清除作用, 而对H2O2无清除作用; 测定了稀土对线粒体ROS代谢酶(谷胱甘肽过氧化物酶和超氧化物歧化酶)的活性影响. 结果表明, 三种稀土离子对线粒体谷胱甘肽过氧化物酶的活性基本没有影响, 而Gd3+和Yb3+稀土离子能明显抑制线粒体超氧化物歧化酶的活性.
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| 关 键 词： | 线粒体 活性氧(ROS) |
| 文章编号： | 0251-0790（2006）06-0999-04 |
| 收稿时间： | 2005-10-25 |
| 修稿时间： | 2005-10-25 |
Effects of Lanthanide Ions(La3+ , Gd3+ , Yb3+ ) on Production of Reactive Oxygen Species in Mitochondria |
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| LIU Hui-Xue,YANG Xiao-Da,WANG Kui. Effects of Lanthanide Ions(La3+ , Gd3+ , Yb3+ ) on Production of Reactive Oxygen Species in Mitochondria[J]. Chemical Research In Chinese Universities, 2006, 27(6): 999-1002 |
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| Authors: | LIU Hui-Xue YANG Xiao-Da WANG Kui |
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| Affiliation: | Department of Chemical Biology, School of Pharmaceutical Sciences, Peking
University, Beijing 100083, China |
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| Abstract: | The effects of lanthanides(Ln) on the production of reactive oxygen species(ROS) in isolated mitochondria were investigated by fluorescence assays upon incubation with Ln3+. The results show that Ln3+(La3+, Gd3+, Yb3+) could reduce the rates of overall ROS production. The results obtained from experiments on xanthine/xanthine oxidative enzyme system suggest that Ln3+ ions can reduce the formation of ·O-2, but H2O2 did not react with Ln3+. The influences of Ln3+ on the activities of metabolize enzymes(GSH-Px and Mn-SOD) indicate that Ln3+ could decrease the activity of Mn-SOD, but could not affect the activity of GSH-Px. The effects of Ln3+ on cellular H2O2 level are discussed. |
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| Keywords: | Lanthanide Mitochondria Reactive oxygen species(ROS) |
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