Mass spectrometric monitoring of 2-oxoglutaric acid in fermentation broth |
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| Authors: | E. Pungor M. Pecs L. Szigeti L. Nyeste J. Szilagyi |
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| Affiliation: | Department of Agricultural Chemical Technology, Technical University, Budapest Hungary;Biogal Pharmaceutical Works, Debrecen Hungary |
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| Abstract: | A mass spectrometric method for monitoring 2-oxoglutarie acid (an essentially nonvolatile compound) in aqueous solutions is described. Silicone rubber membranes are used for mass spectrometric sampling; the acid is esterified with methanol. The assay was applied to monitoring 2-oxoglutarie acid in penicillin fermentation broths. Three ion peaks of the ester were suitable for measurements. Preliminary work on faster esterification with diazomethane in diethyl ether is reported. |
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