Fluorimetric assay for argininosuccinate lyase in human plasma and erythrocytes with a benzoin reagent |
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| Authors: | Masaaki Kai Yosuke Ohkura |
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| Institution: | Faculty of Pharmaceutical Sciences, Kyushu University 62, Maidashi, Higaski-ku, Fukuoka 812 Japan |
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| Abstract: | A fluorimetric method for the assay of argininosuccinate lyase in human plasma (15–150 units) and erythrocytes (40–1200 units) is established. The arginine formed enzymatically is quantified by means of its fluorescent reaction with benzoin. The method is rapid, simple and sensitive enough to allow the enzyme activity to be determined in as little as 100 μl of plasma or 12.5 μl of packed erythrocytes. |
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