Evaluation of a dialysis probe for continuous sampling in fermentors and in complex media |
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Authors: | CF Mandenius B Danielsson B Mattiasson |
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Institution: | Department of Pure & Applied Biochemistry, University of Lund, Box 124, S-221 00 Lund Sweden |
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Abstract: | A dialysis probe is described for continuous sampling from complex solutions, such as fermentation broth, milk or waste water, to yield samples suitable for liquid chromatography, flow injection analysis, enzyme calorimetry, etc. The analyte is transferred to a flow stream separated from the sample by a dialysis membrane that is protected from fouling by a strong tangential flow of the sample solution. This flow is accomplished by placing a magnetic stirring bar close to the membrane surface. The device is constructed of materials permitting the probe to be steam-sterilized when mounted inside a fermentor. |
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