Copper(II) binding to alpha-synuclein, the Parkinson's protein |
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| Authors: | Lee Jennifer C Gray Harry B Winkler Jay R |
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| Institution: | Laboratory of Molecular Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892-8013, USA. leej4@mail.nih.gov |
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| Abstract: | Variations in tryptophan fluorescence intensities confirm that copper(II) interacts with alpha-synuclein, a protein implicated in Parkinson's disease. Trp4 fluorescence decay kinetics measured for the F4W protein show that Cu(II) binds tightly (Kd 100 nM) near the N-terminus at pH 7. Work on a F4W/H50S mutant indicates that a histidine imidazole is not a ligand in this high-affinity site. |
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