Determination of seven arsenic compounds in urine by HPLC-ICP-DRC-MS: a CDC population biomonitoring method |
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| Authors: | Carl P Verdon Kathleen L Caldwell Mark R Fresquez Robert L Jones |
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| Institution: | (1) Inorganic and Radiation Analytical Toxicology Branch, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, N.E., Mail Stop F-50, Atlanta, GA 30341, USA;(2) Battelle Memorial Institute, Atlanta, GA 30341, USA |
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| Abstract: | A robust analytical method has been developed and validated by use of high-performance liquid chromatography inductively coupled plasma mass spectrometry with Dynamic Reaction Cell? (DRC) technology that separates seven arsenic (As) species in human urine: arsenobetaine (AB), arsenocholine, trimethylarsine oxide (TMAO), arsenate (As(V)), arsenite (As(III)), monomethylarsonate, and dimethylarsinate. A polymeric anion-exchange (Hamilton PRP® X-100) column was used for separation of the species that were detected at m/z 75 by ICP-DRC-MS (PerkinElmer? SCIEX® ELAN DRCII?) using 10% hydrogen–90% argon as the DRC gas. The internal standard (As) is added postcolumn via an external injector with a sample loop. All analyte peaks were baseline-separated except AB and TMAO. Analytical method limits of detection for the various species ranged from 0.4 to 1.7 μg L?1 as elemental As. As(III) conversion to As(V) was avoided by adjusting the urine sample to
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| Keywords: | Arsenic Speciation High-performance liquid chromatography Inductively coupled plasma mass spectrometry Urine Biomonitoring |
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