Fluorimetric Determination of Thyroxine Hormone with Eu(III)-(Pyridine-2,6-Dicarboxylate) Tris Complex |
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Authors: | Elmas Gök Serdar Ates |
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Affiliation: | (1) Department of Chemistry, Hacettepe University, 06532 Ankara, Turkey |
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Abstract: | We describe a method for the determination of thyroxine (Thy) using its quenching effect on Eu(PDA)33- tris complex fluorescence. The relative fluorescence intensities are measured at fixed exc = 282 nm, em = 615 nm by titrating the metal complex with Thy in piperazine buffer solution at pH 6.5. Data indicated an associative type of reaction of two molecules valid between 0.0 < R < 1.0, R being the mole ratio of Eu(PDA)33- to Thy. Over this ratio and up to (R 1.0) collisional quenching of Eu(PDA)33- complex ion emission is seen as a result of heavy atom effect, intermolecular energy transfer playing the main role. This is also confirmed by the Stern-Volmer equation. In optimized experimental conditions, the L- form of Thy is determined in a range of 15.5–551.6 g/ml (2.0 × 10–5 – 7.1 × 10–4M) with relative error of ±1.17%. |
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Keywords: | Thyroxine hormone Eu(III) chelate Stern-Volmer Equation heavy atom effect |
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