The Role of Lipids in Allosteric Modulation of Dopamine D2 Receptor—In Silico Study |
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| Authors: | Justyna uk Damian Bartuzi Przemys&#x;aw Miszta Agnieszka A Kaczor |
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| Institution: | 1.Department of Synthesis and Chemical Technology of Pharmaceutical Substances with Computer Modelling Laboratory, Faculty of Pharmacy, Medical University of Lublin, 4A Chodźki St., PL-20093 Lublin, Poland; (J.Ż.); (D.B.);2.Faculty of Chemistry, Biological, Chemical Research Centre, University of Warsaw, PL-02093 Warsaw, Poland;3.School of Pharmacy, University of Eastern Finland, Yliopistonranta 1, P.O. Box 1627, FI-70211 Kuopio, Finland |
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| Abstract: | The dopamine D2 receptor, belonging to the class A G protein-coupled receptors (GPCRs), is an important drug target for several diseases, including schizophrenia and Parkinson’s disease. The D2 receptor can be activated by the natural neurotransmitter dopamine or by synthetic ligands, which in both cases leads to the receptor coupling with a G protein. In addition to receptor modulation by orthosteric or allosteric ligands, it has been shown that lipids may affect the behaviour of membrane proteins. We constructed a model of a D2 receptor with a long intracellular loop (ICL3) coupled with Giα1 or Giα2 proteins, embedded in a complex asymmetric membrane, and simulated it in complex with positive, negative or neutral allosteric ligands. In this study, we focused on the influence of ligand binding and G protein coupling on the membrane–receptor interactions. We show that there is a noticeable interplay between the cell membrane, G proteins, D2 receptor and its modulators. |
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| Keywords: | allosteric modulators dopamine D2L receptor GPCRs lipid rafts molecular dynamics |
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