Exploring laccase-like multicopper oxidase genes from the ascomycete <Emphasis Type="Italic">Trichoderma reesei</Emphasis>: a functional,phylogenetic and evolutionary study |
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| Authors: | Anthony Levasseur Markku Saloheimo David Navarro Martina Andberg Pierre Pontarotti Kristiina Kruus Eric Record |
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| Institution: | 1.UMR-1163, INRA de Biotechnologie des Champignons Filamenteux, IFR86-BAIM,Universités de Provence et de la Méditerranée, ESIL,Marseille Cedex 09,France;2.Universités Aix-Marseille 1 et 2, UMR-1163,Marseille Cedex 09,France;3.VTT Biotechnology: VTT Technical Research Centre of Finland,Espoo,Finland;4.Evolution Biologique et Modélisation: UMR 6639 CNRS Université de Aix Marseille,Marseille,France |
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| Abstract: | Background The diversity and function of ligninolytic genes in soil-inhabiting ascomycetes has not yet been elucidated, despite their
possible role in plant litter decay processes. Among ascomycetes, Trichoderma reesei is a model organism of cellulose and hemicellulose degradation, used for its unique secretion ability especially for cellulase
production. T. reesei has only been reported as a cellulolytic and hemicellulolytic organism although genome annotation revealed 6 laccase-like
multicopper oxidase (LMCO) genes. The purpose of this work was i) to validate the function of a candidate LMCO gene from T. reesei, and ii) to reconstruct LMCO phylogeny and perform evolutionary analysis testing for positive selection. |
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