PHOTOCHEMICAL LABELING OF YEAST ALCOHOL DEHYDROGENASE WITH AN AZIDE ANALOG OF NAD + |
| |
| Authors: | Stephen S. Hixson Susan H. Hixson |
| |
| Affiliation: | Chemistry Department, University of Massachusetts, Amherst, Massachusetts 01002, U.S.A. |
| |
| Abstract: | Abstract— 3-Azidopyridine adenine dinucleotide, an azide analog of NAD+, has been synthesized as a potentially general photochemical labeling reagent for the active sites of NAD-dependent dehydrogenases. The analog is a competitive inhibitor of NAD reduction by yeast alcohol dehydrogenase (YADH). Upon photolysis of the 3H-analog in the presence of YADH, 7% of the label becomes covalently bound. The results are discussed in terms of desired properties of a photochemical labeling reagent. |
| |
| Keywords: | |
|
|
