Gas chromatography - optical fiber detector for assessment of fatty acids in urban soils |
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Authors: | Silva Lurdes Cachada Anabela Pereira Ruth Freitas Ana Cristina Rocha-Santos Teresa A P Panteleitchouk Teresa S L Pereira Maria E Duarte Armando Costa |
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Affiliation: | a CESAM (Centre for Environmental and Marine Studies) & Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal b CESAM (Centre for Environmental and Marine Studies) & Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal c ISEIT/Viseu, Instituto Piaget, Estrada do Alto do Gaio, Galifonge, 3515-776 Lordosa, Viseu, Portugal |
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Abstract: | Fatty acids have been used as biomarkers of the microbial community composition of soils and they are usually separated and quantified by gas-chromatography coupled to a flame ionization detector (GC-FID). The aim of this study was to develop, validate and apply a methodology based on gas chromatography coupled to optical fiber detection (GC-OF) for screening five fatty acids used as indicators of fungal and bacterial communities in urban soils. The performance of the GC-OF methodology (optical fiber detector at 1550 nm) was evaluated by comparison with the GC-FID methodology and it was found that they were comparable in terms of linear range, detection limit and analytical errors. Besides these similar analytical characteristics, the GC-OF is much cheaper than the GC-FID methodology. Different concentrations were determined for each fatty acid indicator which in turn varied significantly between the soil samples analyzed from Lisbon ornamental gardens. Additionally, the GC-OF showed a great potential as alternative for determination of eleven or more fatty acids in urban soils. |
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Keywords: | Fatty acids GC-OF GC-FID Urban soil |
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