Uronic acid determination by high performance liquid chromatography with postcolumn fluorescence derivatization |
| |
| Authors: | Kakita Hirotaka Kamishima Hiroshi Inouye Kuniyo |
| |
| Institution: | AIST Shikoku, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Kagawa, Japan. h-kakita@aist.go.jp |
| |
| Abstract: | To develop a fluorimetric high performance liquid chromatography (HPLC) technique for uronic acid microanalysis, a saline mobile phase and the postcolumn fluorimetric determination were combined. The detection limits of D-glucuronic, D-galacturonic and D-mannuronic acids were 7.19, 23.88 and 7.08 pmol, respectively. The proposed method was successfully applied to uronic acid microanalysis in a polysaccharide hydrolysate and a drink. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
