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In Situ Scatheless Cell Detachment Reveals Correlation between Adhesion Strength and Viability at Single‐Cell Resolution |
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| Authors: | Sifeng Mao Wanling Zhang Qiushi Huang Mashooq Khan Haifang Li Prof Katsumi Uchiyama Jin‐Ming Lin |
| Institution: | 1. Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, China;2. Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, Hachioji, Tokyo, Japan |
| Abstract: | Single‐cell biology provides insights into some of the most fundamental processes in biology and promotes the understanding of life's mysteries. As the technologies to study single‐cells expand, they will require sophisticated analytical tools to make sense of various behaviors and components of single‐cells as well as their relations in the adherent tissue culture. In this paper, we revealed cell heterogeneity and uncovered the connections between cell adhesion strength and cell viability at single‐cell resolution by extracting single adherent cells of interest from a standard tissue culture by using a microfluidic chip‐based live single‐cell extractor (LSCE). We believe that this method will provide a valuable new tool for single‐cell biology. |
| Keywords: | analytical methods cell adhesion intracellular metabolites live single-cell extraction microfluidic chips |