Development and application of an integrated system for monitoring ethanol content of fuels |
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Authors: | Eliana M Alhadeff Andrea M Salgado Nei Pereira Jr Belkis Valdman |
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Institution: | 1. Departamento de Engenharia Química, Escola de Química, CT/UFRJ, Ilha do Fund?o, Cidade Universitária, CEP 21.949.900, Rio de Janeiro, RJ, Brasil
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Abstract: | An automated flow injection analysis (FIA) system for quantifying ethanol was developed using alcohol oxidase, horseradish
peroxidase, 4-aminophenazone, and phenol. A colorimetric detection method was developed using two different methods of analysis,
with free and immobilized enzymes. The system with free enzymes permitted analysis of standard ethanol solution in a range
of 0.05–1.0 g of ethanol/L without external dilution, a sampling frequency of 15 analyses/h, and relative SD of 3.5%. A new
system was designed consisting of a microreactor with a 0.91-mL internal volume filled with alcohol oxidase immobilized on
glass beads and an addition of free peroxidase, adapted in an FIA line, for continued reuse. This integrated biosensor-FIA
system is being used for quality control of biofuels, gasohol, and hydrated ethanol. The FIA system integrated with the microreactor
showed a calibration curve in the range of 0.05–1.5 g of ethanol/L, and good results were obtained compared with the ethanol
content measured by high-performance liquid chromatography and gas chromatography standard methods. |
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Keywords: | Biosensors gasohol immobilized enzymes alcohol oxidase horseradish peroxidase |
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