酪氨酸微波水热法制备碳点及其在Fe3+检测和荧光标记方面的应用

以酪氨酸为原料，乙二胺为钝化剂，采用绿色、简便的微波辅助水热法制备了尺寸均一、分散性好的碳点.通过调节乙二胺的加入量可以在一定程度上对碳点尺寸进行调节.所得碳点可以通过改变激发波长实现蓝光到绿光的转变，且荧光强度随激发光波长的红移也保持了一定的强度.所得碳点对Fe³⁺具有很好的选择性猝灭，被用于检测Fe³⁺时检测限为0.82 nmol/L，检出限为2.98 nmol/L.所制备碳点具有良好的生物相容性和低细胞毒性，被用于标记AD293细胞时标记位置为细胞质.

Microwave-assisted Hydrothermal Synthesis of Carbon Dots Based on Tyrosine and Their Application in Ion Detection and Bioimaging

Cabon dots(CDs) were synthesized utilizing tyrosine as carbon source, ethanediamine as passivant through a green microwave-assisted hydrothermal way. The size of CDs can be adjusted by the dose of ethanediamine. The as-prepared CDs have uniform size and good dispersibility, showing blue or green fluorescence. The as-prepared CDs can change their fluorescence(from blue to yellow) by changing the excitation light and the emission light can be kept without large decay. The as-synthesized CDs have good selectivity on the detection of Fe³⁺ with a limit of detection(LOD) of 0.82 nmol/L, and a detection limit of 2.98 nmol/L. The as-synthesized CDs also have great biocompatibility and low biotoxicity, and can be utilized as a probe to label AD293 cells, marking the cytoplasm of the cells.