基于镍丝负载氧化镍纳米片的尿酸生物传感器

利用水热法与高温热处理相结合, 在金属镍丝表面制备了氧化镍纳米片, 这些纳米片相互交错, 均匀覆盖于镍丝表面, 其宽度约为数百纳米, 厚度约10 nm. 将镍丝负载氧化镍纳米片吸附固定尿酸氧化酶后, 得到尿酸生物传感器电极, 该电极显示出优异的电化学性能, 其灵敏度达到821.4 μA/(mmol·cm²), 线性检测范围为1~900 μmol/L, 检出限为0.1 μmol/L, 同时具有良好的抗干扰特性. 该尿酸传感器电极易于进行植入式探测或与微流控技术相结合, 为快速、 高灵敏的尿酸检测提供了新途径.

Uric Acid Biosensor Based on Ni Wire Modified with NiO Nanosheets†

Ni wire modified with NiO nanosheets was synthesized by hydro-thermal method accompanied with heat treatment at high-temperature. The characterized results revealed that these NiO nanosheets grew uniformly on the surface of the Ni wire, with a size of several hundreds of nanometers and a thickness of about ten nanometers. Electrochemical experiments showed that the Ni wire modified with NiO nanosheets could be used as a kind of good material for immobilization of uricase to fabricate an efficient uric acid biosensor. Sensitivity of the prepared biosensor was found to be 821.4 μA/(mmol·cm²). The linear range for the detection was 1—900 μmol/L. The detection limit was 0.1 μmol/L. These results indicate that the Ni wire modified with NiO nanosheets is a new platform for the construction of biosensors.