柳叶五层龙正丁醇提取部分中的3个新酚性成分及其α-葡萄糖苷酶抑制活性

采用多种色谱分离方法从柳叶五层龙(Salacia cochinchinensis Lour)的茎叶中分离得到5个化合物. 通过一维(1D)及二维核磁共振波谱(2D NMR), 包括核磁共振氢谱(¹H NMR)、 核磁共振碳谱(¹³C NMR)、 异核单量子相关(HSQC)、 异核多键相关(HMBC)、 氢氢相关(¹H-¹H COSY)和旋转坐标中的欧沃豪斯增强光谱(ROESY), 以及红外光谱(IR)和电喷雾电离高分辨质谱(ESI-HRMS)等方法, 鉴定其结构分别为Salaciacochinoside A(1), 5'-O-3,4,5-trimethoxybenzoyl-β-D-apiofuranoside(2), 5-methoxy-anticerol A(3), 21α,30-dihydroxy-D∶A-friedooleanan-3-one(4)和21α,26-dihydroxyfriedelan-3-one(5). 化合物1~3为新化合物. α-葡萄糖苷酶抑制活性测试结果显示, 化合物1和3对α-葡萄糖苷酶具有显著的抑制作用, 其IC₅₀值分别为0.32和0.59 μmol/L; 化合物2, 4和5未表现出α-葡萄糖苷酶抑制活性.

Three New Phenolic Compounds from Salacia cochinchinensis Lour and Their α-Glucosidase Inhibitory Activities†

Five compounds were isolated from the stems and leaves of Salacia cochinchinensis Lour. By comprehensive spectroscopic analyses including mass spectroscopy(MS), infrared radiation(IR), 1D and 2D nuclear magnetic resonance(NMR) heteronuclear single quantum coherence(HSQC), heteronuclear multiple bond correlation(HMBC), ¹H-¹H chemical-shift correlation spectroscopy(¹H-¹H COSY) and rotating frame overhauser effect spectroscopy(ROESY)], their structures were elucidated as Salaciacochinoside A(1), 5'-O-3,4,5-trimethoxybenzoyl-β-D-apiofuranoside(2), 5-methoxy-anticerol A(3), 21α,30-dihydroxy-D∶A-friedooleanan-3-one(4) and 21α,26-dihydroxyfriedelan-3-one(5), respectively. Compounds 1—3 were new compounds. All the compounds were assayed for the anti-diabetic activities. Compounds 1 and 3 exhibited remarkable bioactivity, inhibiting α-glucosidase with IC₅₀ values of 0.32 and 0.59 μmol/L, respectively. Compounds 2, 4 and 5 showed none anti-diabetic activity in current experiment.