过度训练动物模型的建立及过度训练状态下大鼠肠粘膜病理性变化的研究

为阐明过度训练状态下肠粘膜超微结构和免疫的病理性变化及其发生机制,采用建立一般训练和过度训练大鼠模型,应用形态学手段和分子生化技术,结合免疫组化和图像分析方法,对训练后两组大鼠肠道粘膜的超微结构、脂质过氧化反应产物丙二醛（MDA）、还原型谷胱甘肽（GSH）、超氧化物歧化酶（SOD）和粘膜IgA的产生及含量作定位和定量研究.结果表明,第4周末,一般训练组和过度训练组大鼠肠道粘膜的超微结构、MDA和GSH含量、SOD活性和粘膜IgA水平未见异常改变（p>0.05）.但是第8周末过度训练组电境下见肠道粘膜超微结构明显改变,MDA含量明显升高,GSH含量和SOD活性明显降低;第8周末,IgA浆细胞计数及其平均吸光度,以及粘液IgA水平均显著低于对照组（p<0.01）.结果提示过度训练可以引起肠道粘膜超微结构和IgA屏障结构改变,这些改变可能与肠粘膜自由基损伤有关.

Establishing Overtraining Model and Investigation on Effectsof Overtraining on the Ultrastructure and Immunity of Intestinal Mucosa in Rats

To investigate the effects of overtraining on the ultrastructure and lipid peroxidation level of intestinal mucosa and mucosal IgA immunity and its mechanisms, electron microscope, immunohistochemical technique and image analysis were used in overtraining group (OG) and control group(CG) rats. There was no abmormal changes on the ultrastructure and lipid peroxidation level of intestinal mucosa and mucosal IgA immunity and its mechanisms in OG and CG at the end of 4 weeks, but the ultrastructure was seriously destroyed and the MDA content was significant increnase in OG at the end of 8 weeks. The number of IgA-containing cells and their mean optical density (A) and the level of mucus IgA were all significantly lower in OG than in CG at the end of 8 weeks (p<0.01). It is concluded that overtrainiong could exert effects on the mucosa and mucus IgA barrier of intestine.