Light-initiated hydroxylation of lauric acid using hybrid P450 BM3 enzymes |
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| Authors: | Tran Ngoc-Han Huynh Ngoc Bui Thuba Nguyen Yen Huynh Phuong Cooper Mary E Cheruzel Lionel E |
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| Institution: | San José State University, Department of Chemistry, One Washington Square, San José, CA 95192-0101, USA. |
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| Abstract: | We have developed hybrid P450 BM3 enzymes consisting of a Ru(II)-diimine photosensitizer covalently attached to non-native single cysteine residues of P450 BM3 heme domain mutants. These enzymes are capable, upon light activation, of selectively hydroxylating lauric acid with 40 times higher total turnover numbers compared to the peroxide shunt. |
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