| 有核细胞的弹性光散射模型 |
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| 引用本文： | 吴大建,王亚伟,韩广才.有核细胞的弹性光散射模型[J].光学学报,2005,25(12):670-1675. |
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| 作者姓名： | 吴大建 王亚伟 韩广才 |
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| 作者单位： | 江苏大学理学院,镇江,212013;江苏大学理学院,镇江,212013;江苏大学理学院,镇江,212013 |
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| 基金项目： | 中国博士后科学基金（2004035656）和江苏省教育厅自然基金（04kjb140017）资助课题. |
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| 摘 要： | 提出一种新型的同心椭球构成的同心椭球（CEM）模型来模拟有核细胞，以瑞利-德拜-甘斯（Rayleigh-Debye-Gans）近似理论为基础，对细胞内不同部分的传播常量加以修正，用该模型代替球系列模型，研究其光散射特性。结果表明同心椭球模型更精确地反映了非球形有核细胞光散射的真实情况，同时其前向、侧向及后向光散射精细谱的呈现，可以在庞杂的细胞中辨别不同的细胞类型，较好的应用于细胞的变形性研究和细胞分类研究的领域。
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| 关 键 词： | 医用光学与生物 光散射 细胞 椭球体 形体因子 |
| 文章编号： | 0253-2239（2005）12-1670-6 |
| 收稿时间： | 2004-12-22 |
| 修稿时间： | 2005-04-10 |
Model for Elastic Light Scattering from Nucleated Cells |
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| Wu Dajian,Wang Yawei,Han Guangcai.Model for Elastic Light Scattering from Nucleated Cells[J].Acta Optica Sinica,2005,25(12):670-1675. |
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| Authors: | Wu Dajian Wang Yawei Han Guangcai |
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| Institution: | Institute of Science, Jiangsu University, Zhenjiang 212013 |
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| Abstract: | A model is presented to calculate the light scattering properties of nucleated cells which are mimiced by two concentric ellipsoids(CEM).The light-scattering characteristics are explored,deriving from the Rayleigh-Debye-Gans approximation and by simultaneous application of field averaging of the internal field and modification of the propagation constant inside the different cellular compartments.It is shown that the CEM is superior to the ball model.CEM can reflect the true configuration of the nonspherical nucleated cells more accurately.In addition,the simultaneous detection of the light-scattering intensities in the forward-,lateral-,and back-scattering directions can help distinguish the different cell types in heterogeneous population of nucleated cells,so it can be well used in cell classification and research of cell transfiguration. |
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| Keywords: | medical optics biotechnology light-scattering cell ellipsoid body factor |
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