大黄牡丹汤组方对急性胰腺炎模型大鼠炎症反应及氧化应激水平的影响

The Effect of Dahuang Mudan Decoction on Inflammatory Response and Oxidative Stress in Acute Pancreatitis Model Rats

Abstract: Objective To study the effect of Dahuang Mudan Decoction ( RPDP) on inflammatory response and oxidative stress in acute pancreatitis( AP) model rats． Method Divided 90 SD rats randomly into three groups: the sham operation( SO) group ，the AP group，and the RPDP group． The models of AP were made by pressurize inject 3% sodium taurocholate into the bile duct where was 0.3mm away from the pancreatic duct with the speed of 0. 1ml /min． The rats in the RPDP group were lavaged with RPDP ( 40 g /kg) after made AP model． The rats in the SO group and the AP group were lavaged with partes aequales Sodium Chloride． Sacrifice the rats 4 h，8 h，12 h hours after establish the animal model，separated the serum and detected the content of IL-6，IL-β and TNF-α in the serum，cut pancreatic tissue in every time quantum and detected the content of MDA，MPO and SOD． Result Compared with the AP group，the serum content of IL-6，IL-β and TNF-α were significantly decrease in RPDP group( P ＜ 0. 05) ，the content of MDA and MPO in pancreatic tissue in every time quantum were also decrease in RPDP group( P ＜ 0. 05) ，while the content of SOD were significantly improve( P ＜ 0. 05) ． Conclusion Dahuang Mudan Decoction could repress the release of TNF-α，IL-6 and IL-β，could decrease the product of MDA，and had the effect of anti-oxidative damage，cleaning oxygen free radicals．