| 氯乙基亚硝基脲导致DNA股间交联的荧光光谱法研究 |
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| 引用本文： | 任婷,赵丽娇,唐巍,钟儒刚. 氯乙基亚硝基脲导致DNA股间交联的荧光光谱法研究[J]. 分析测试学报, 2010, 29(2). DOI: 10.3969/j.issn.1004-4957.2010.02.001 |
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| 作者姓名： | 任婷 赵丽娇 唐巍 钟儒刚 |
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| 作者单位： | 北京工业大学,生命科学与生物工程学院,北京,100124 |
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| 基金项目： | 国家自然科学基金资助项目，北京市自然科学基金资助项目，北京优秀人才培养资助项目 |
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| 摘 要： | 氯乙基亚硝基脲(CENUs)是重要的临床抗癌药物,其抗癌作用机制与导致DNA股间交联密切相关。使用荧光光谱法对其导致的DNA股间交联进行定量分析。结果表明,交联率随着反应时间的延长逐渐增加,司莫司汀(Me-CCNU)和卡莫司汀(BCNU)的交联率分别约在8 h和5 h达到最大值,且药物浓度越大,交联率增加越快。比较了Me-CCNU和BCNU对DNA交联作用的反应动力学,发现分解较慢的Me-CCNU与DNA的交联过程中存在一段明显的"诱导期";而分解较快的BCNU与DNA的交联反应则不存在"诱导期",且BCNU浓度过高或反应时间过长均会使交联率下降。该文为阐明CENUs导致DNA交联的反应动力学和反应机理提供了依据。
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| 关 键 词： | 氯乙基亚硝基脲 DNA股间交联 荧光光谱分析 反应动力学 |
Agarose Gel Electrophoresis and Fluorometric Study on DNA Interstrand Cross-links Induced by Chlorethylnitrosoureas |
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| REN Ting,ZHAO Li-jiao,TANG Wei,ZHONG Ru-gang. Agarose Gel Electrophoresis and Fluorometric Study on DNA Interstrand Cross-links Induced by Chlorethylnitrosoureas[J]. Journal of Instrumental Analysis, 2010, 29(2). DOI: 10.3969/j.issn.1004-4957.2010.02.001 |
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| Authors: | REN Ting ZHAO Li-jiao TANG Wei ZHONG Ru-gang |
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| Affiliation: | REN Ting,ZHAO Li-jiao,TANG Wei,ZHONG Ru-gang(College of Life Science , Bioengineering,Beijing University of Technology,Beijing 100124,China) |
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| Abstract: | Chlorethylnitrosoureas(CENUs) are significant anticancer drugs in the clinical treatment of human malignancies.Several lines of evidences indicated that their anticancer mechanism was related to the formation of DNA interstrand cross-links(ICLs).Quantitative analyses were carried out to investigate the DNA ICLs using spectrofluorometric methods.The results showed that the cross-link fraction(CF%) increased progressively accompanied with reaction time.The CF% reached the maximum value when DNA had been treat... |
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| Keywords: | chlorethylnitrosoureas DNA interstrand cross-links spectrofluorimetric analysis reaction kinetics |
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