| 植物生长调节物质对华黄芪愈伤组织诱导、增殖及芽分化的效应 |
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| 引用本文： | 董诚明,许桂芳,周吉源,赵洁,熊丽.植物生长调节物质对华黄芪愈伤组织诱导、增殖及芽分化的效应[J].华中师范大学学报(自然科学版),1994,33(4):0-0. |
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| 作者姓名： | 董诚明 许桂芳 周吉源 赵洁 熊丽 |
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| 作者单位： | 河南中医学院中药系 |
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| 摘 要： | 以华黄芪叶片为外植体，在附加不同浓度和比例的２，４－Ｄ与６－ＢＡ的ＭＳ培养基上，探讨植物生长调节物质对华黄芪愈伤组织诱导、增殖及芽分化的效应，并在愈伤组织增殖过程中，初步对愈伤指数与愈伤组织生长量的关系进行了比较，结果表明：在ＭＳ＋２，４－Ｄ０．５ｍｇ／Ｌ＋６－ＢＡ０．３ｍｇ／Ｌ培养基上诱导率最高，且形成愈伤组织时间最短；在ＭＳ＋２，４－Ｄ０ｍｇ／Ｌ＋６－ＢＡ０．３ｍｇ／Ｌ培养基上，芽的分化率最高；愈伤指数与“称重法”测定愈伤组织生长所得结果一致．
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| 关 键 词： | 植物生长调节物质 愈伤组织 分化 华黄芪 |
| 收稿时间： | 1994-04-25 |
EFFECTS OF PLANT GROWTH REGULATORS ON CALLUS INDUCTION,GROWTH AND BUD DIFFERENTIATION OF ASTRAGALUS CHINENSIS L. |
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| Dong Chengming,Xu Guifang,Zhou Jiyuan,Zhao Jie,Xiong Li.EFFECTS OF PLANT GROWTH REGULATORS ON CALLUS INDUCTION,GROWTH AND BUD DIFFERENTIATION OF ASTRAGALUS CHINENSIS L.[J].Journal of Central China Normal University(Natural Sciences),1994,33(4):0-0. |
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| Authors: | Dong Chengming Xu Guifang Zhou Jiyuan Zhao Jie Xiong Li |
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| Abstract: | Segments of leaf of Astragalus chinansis L., cultired in vitro on MS medium supplenmented wity different plant growth regulators,its callus induction,growth and bud differentiation can be different. Supplenmented with 2, 4-D 0. 5 mg/L + 6-BA0. 3 mg/L,the percentage of callus induction is the highest, while supplenmented with 2, 4-D0 mg/L + 6-BA0. 3 mg/L, bud differentiat most." callus parameter"is used in place of callus biomass for for first time. |
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| Keywords: | plant growth regulator callus differentiation Astragalus chinensis L |
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