Microwave-Assisted Aqueous Synthesis of Highly Luminescent Carboxymethyl Chitosan-Coated CdTe/CdS Quantum Dots as Fluorescent Probe for Live Cell Imaging |
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Authors: | Zhenyu He Honghao Zhu Peijiang Zhou |
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Institution: | (1) College of Resource and Environmental Science, Wuhan University, Wuhan, 430079, Hubei Province, People’s Republic of China;(2) Wuhan Centers for Disease Prevention and Control, Wuhan, 430022, Hubei Province, People’s Republic of China; |
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Abstract: | This paper describes the development of a simplified and rapid method for the aqueous synthesis of quantum dots (QDs) with
CdTe cores and gradient CdS external shells (CdTe/CdS QDs) aided by microwave irradiation. Several synthesis parameters, such
as molar ratio of reagents, pH, reaction temperature, and reaction time, were studied in details. Under the optimized conditions,
highly effective CdTe/CdS QDs could be synthesized in aqueous phase in only 15 min. In order to improve the biocompatibility
of the CdTe/CdS QDs, these QDs were then interacted with carboxymethyl chitosan (CMC) so as they could be used as fluorescent
probes in the aqueous phase. With the incorporation of CMC, the stability of modified QDs was found to have improved significantly
(from 4 months to more than 10 months at room temperature). The photoluminescence quantum yield (PLQY) of the modified QDs
could reach 75%, other parameters include a full width at half maximum of the emission (FWHM) spectrum as 40 ~ 60 nm, and
an average size, estimated from electron microscopic images, as 3.5 nm. As fluorescent probes, these modified QDs were successfully
used for imaging live Madin–Darby canine kidney (MDCK) cells, in which the preliminary results indicated that these modified
QDs demonstrated good biocompatibility and showed promising applications for bio-labeling and imaging. |
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