Solid-phase microextraction for the investigation of sex pheromone of Eucosma notanthes Meyrick

A simple and efficient technique that does not require solvent and uses less operating time for the investigation of sex pheromones of the carambola fruit borer (Eucosma notanthes Meyrick) by utilizing headspace solid-phase microextraction (SPME) followed by GC-MS analysis has been developed. Variables such as types of SPME fiber, number of pests, temperature and extraction time have been studied. Whole sex glands of Eucosma notanthes Meyrick were dissected from 5 virgin insects, placed in a 2 mL vial, equilibrated at 170 °C for 10 min, and then extracted by headspace SPME at room temperature for 5 min. The results of the GC-MS analyses of headspace SPME of these sex glandular solid samples were much better than those obtained with hexane extraction of sex glandular from 117 insects followed by either headspace SPME or direct injection due to higher absorption efficiency. The simplicity of this technique renders it a very suitable method for research on the biological control of pests.