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Immuno-arrays for multianalyte analysis of chlorotriazines
Authors:Bhand Sunil  Surugiu Ioana  Dzgoev Anatoly  Ramanathan Kumaran  Sundaram P V  Danielsson Bengt
Institution:a Pure and Applied Biochemistry, Lund University, Box 124, S-22100 Lund, Sweden
b PROMED, The Voluntary Health Services, Adyar, Chennai 600113, India
Abstract:In this paper, a novel strategy for multicomponent analysis of two classes of pesticides such as triazines (atrazine and simazine) and phenoxyalkanoic acids (2,4-dichlorophenoxy acetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), 4-chlorophenoxyacetic acid (CPOAc), phenoxyacetic acid (POAc)) employing immuno-arrays is demonstrated. The approach is based on cross-reactive arrays of specific antibody pairs coupled to chemometric pattern recognition. The monoclonal antibody pairs employed in this work (atrazine-simazine and 2,4-D) are specific towards a set of analytes and preclude a particular set of others present in the sample matrix. Antibody pairs of atrazine, simazine, and 2,4-D are used to discriminate and quantify analyte of interest. Atrazine was quantified in presence of trace concentration of simazine and that of 2,4-D. The combinatorial cross-reactivity of antibody pairs towards simazine, atrazine and 2,4-D is used to distinguish among different classes of analytes and their influence on the signal suppression in immuno-techniques. These sensors exclude recognition by carbamates such as carbaryl and carbofuran.
Keywords:Multicomponent analysis  Immunosensor  Cross-reactive arrays  Triazines  Phenoxyalkanoic acids  Discriminative detection
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