A validated solid-liquid extraction method for the HPLC determination of polyphenols in apple tissues Comparison with pressurised liquid extraction |
| |
| Authors: | Alonso-Salces Rosa M Barranco Alejandro Corta Edurne Berrueta Luis A Gallo Blanca Vicente Francisca |
| |
| Affiliation: | Departamento de Química Analítica, Facultad de Ciencia y Tecnología, Universidad del País Vasco/Euskal Herriko Unibertsitatea, P.O. Box 644, E-48080 Bilbao, Spain |
| |
| Abstract: | A solid-liquid extraction procedure followed by reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with a photodiode array detector (DAD) for the determination of polyphenols in freeze-dried apple peel and pulp is reported. The extraction step consists in sonicating 0.5 g of freeze-dried apple tissue with 30 mL of methanol-water-acetic acid (30:69:1, v/v/v) containing 2 g of ascorbic acid/L, for 10 min in an ultrasonic bath. The whole method was validated, concluding that it is a robust method that presents high extraction efficiencies (peel: >91%, pulp: >95%) and appropriate precisions (within day: R.S.D. (n = 5) <5%, and between days: R.S.D. (n = 5) <7%) at the different concentration levels of polyphenols that can be found in apple samples. The method was compared with one previously published, consisting in a pressurized liquid extraction (PLE) followed by RP-HPLC-DAD determination. The advantages and disadvantages of both methods are discussed. |
| |
| Keywords: | ACY, anthocyanins AVI, avicularin CAT, (+)-catechin CAT-2, unknown flavan-3-ol CG-1, unknown anthocyanin CQA, 5-caffeoylquinic acid DHC, dihydrochalcones EC, (&minus )-epicatechin FA, flavan-3-ols FO, flavonols HCA, hydroxycinnamic acids HYP, hyperoside IDE, ideain IQC, isoquercitrin PB2, procyanidin B2 PCM, p-coumaric acid PCQ, 4-p-coumaroylquinic acid PLD-1, hydroxyphloretin diglycoside PLD-2, hydroxyphloretin monoglycoside PLG, phloridzin PLXG, phloretin-2&prime -O-xyloglucoside QCI, quercitrin QG-1, unknown quercetin glycoside RUT, rutin GM, Geza miñ a MK, Moko MN111, Manttoni 111 MNEM7, Manttoni EM7 TX, Txalaka UR, Urdin DAD, diode array detector DW, dried weight HPLC, high-performance liquid chromatography LOD, limit of detection LOQ, limit of quantitation nd, not detected PLE, pressurized liquid extraction PPO, polyphenoloxidase R.S.D., relative standard deviation S.D., standard deviation SLE, solid-liquid extraction |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
