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A simple method for the study of salbutamol pharmacokinetics by ion chromatography with direct conductivity detection |
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| Authors: | Ouyang Jin Duan Jing Li Baeyens Willy R G Delanghe Joris R |
| Institution: | a Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium b Department of Chemistry, Beijing Normal University, Beijing 100875, PR China c Department of Pharmacy, Peking University Third Hospital, Beijing 100083, PR China d Department of Clinical Chemistry, Microbiology and Immunology, University Hospital, De Pintelaan 185, B-9000 Ghent, Belgium |
| Abstract: | A simple method for the study of the pharmacokinetics of salbutamol using ion chromatography with direct conductivity detection without chemical suppression is presented in this paper. Baseline separation of salbutamol from plasma components was achieved by using diluted HNO3 (2 mmol l−1) as mobile phase with acetonitrile (6%, v/v) as modifier. Atenolol was utilized as internal standard. Under the optimal conditions, the linear regression coefficients of the calibration curves are 0.996 within the concentration range of 1000-3 ng ml−1 salbutamol. The detection limit (signal-to-noise ratio = 3) was 1 ng ml−1 salbutamol. The noncompartmental pharmacokinetic parameters for salbutamol following administration of 8 mg salbutamol to 18 subjects were tested. The results coincided most satisfactorily with the results obtained by using reversed-phase high performance liquid chromatography (HPLC) with fluorescence detection. |
| Keywords: | Salbutamol Ion chromatography Pharmacokinetics |
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