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Electrogeneration of polypyrrole/alginate films for immobilization of glucose oxidase
Authors:Chen Shuangjun  Chen Wei  Xue Gi
Affiliation:Department of Polymer Science and Engineering, State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, China.
Abstract:Electrogenerated PPy doped with pSA was used as a substrate for immobilization of GOD. This was achieved via covalent bonding of carboxyl groups of the main chain of alginate with amino groups of the enzyme. The pH-induced aggregation behavior of SA in aqueous solution was employed to provide optimum conditions for electrochemical preparation of PPy by galvanostatic methods. GOD was attached to the electrode surface by reaction between the carboxyl groups in the main chain of pSA with amino groups of GOD after treatment with EDC and NHS. The linkage of GOD enzyme to the conductive surface was characterized by ATR spectroscopy and SEM CV was used to demonstrate the bioactivity of the enzyme electrode toward glucose.
Keywords:electrogeneration  glucose oxidase  immobilization  polypyrrole  protonation  sodium alginate
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