Bio-sensing using recessed gold-filled capillary amperometric electrodes |
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Authors: | A Kacanovska Z Rong M Schmidt P St J Russell P Vadgama |
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Institution: | (1) Queen Mary University of London, Mile End Road, London, E1 4NS, UK;(2) Max Planck Institute for the Science of Light, 91058 Erlangen, Germany; |
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Abstract: | A novel recessed electrode is reported for amperometric detection of hydrogen peroxide and via glucose oxidase for the detection of glucose. The electrode utilised electrodeposited platinum over a gold wire surface,
which proved to be an effective peroxide-detecting surface. Compared with a traditional exposed electrode surface, the recessed
tip facilitated an extended linear range for glucose from 4 to over 14 mM. Bio-fouling, as assessed by exposure to bovine
serum albumin, was also significantly reduced. Though response time at the recess was increased, it was within an acceptable
range for physiological monitoring. Moreover, the recess enabled precise measurement of the hydrogen peroxide diffusion coefficient;
this was based on a bipartite expression for the transient amperometric current at the recessed structure following a step
change in ambient hydrogen peroxide concentration. An important aspect of the diffusion measurement was the curve fitting
routine used to map on to the theoretical response curve. |
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