| 无泵负压进样-芯片电泳分离-激光诱导荧光光谱法测定儿茶酚胺类物质 |
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| 引用本文： | 胡红美,王修中,徐春秀,殷学锋,孙秀梅,郭远明.无泵负压进样-芯片电泳分离-激光诱导荧光光谱法测定儿茶酚胺类物质[J].理化检验(化学分册),2012(4):377-380. |
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| 作者姓名： | 胡红美 王修中 徐春秀 殷学锋 孙秀梅 郭远明 |
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| 作者单位： | 浙江省海洋水产研究所海水增养殖重点实验室;浙江大学化学系微分析系统研究所;青岛农业大学化学与药学院;韩山师范学院化学系 |
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| 基金项目： | 广东省自然科学基金项目(S2011040002246) |
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| 摘 要： | 提出了用芯片电泳分离-激光诱导荧光光谱法测定儿茶酚胺类物质的方法。采用自制的无泵负压进样系统,避免了进样歧视效应。在优化的条件下,去甲肾上腺素(NE)、多巴胺(DA)和肾上腺素(E)可在1 min内完全分离。3种儿茶酚胺的平均迁移时间依次为30.59,37.23,46.43 s,其相对标准偏差(n=7)依次为1.10%,1.28%,0.45%。3种物质的线性范围为0.3～5.0 mg.L-1(NE及DA)和0.05～4.0 mg.L-1(E),检出限(3S/N)依次为30,30,10μg.L-1。
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| 关 键 词： | 芯片电泳 激光诱导荧光光谱法 儿茶酚胺类物质 |
Separation and Determination of Catecholamines by Microchip Electrophoresis with Laser Induced Fluorescence Spectrometry and Pump-free Negative Pressure Sample Introduction |
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| HU Hong-mei,WANG Xiu-zhong,XU Chun-xiu,YIN Xue-feng,SUN Xiu-mei,GUO Yuan-ming.Separation and Determination of Catecholamines by Microchip Electrophoresis with Laser Induced Fluorescence Spectrometry and Pump-free Negative Pressure Sample Introduction[J].Physical Testing and Chemical Analysis Part B:Chemical Analgsis,2012(4):377-380. |
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| Authors: | HU Hong-mei WANG Xiu-zhong XU Chun-xiu YIN Xue-feng SUN Xiu-mei GUO Yuan-ming |
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| Institution: | 1(1.Zhejiang Provincial Key Lab.of Mariculture and Enhancement,Zhejing Provincial Research Institute of Marine Fishery,Zhoushan 316100,China; 2.Institute of Microanalytical Systems,Zhejiang University,Hangzhou 310027,China; 3.College of Chemistry and Pharmacy,Qingdao Agricultural University,Qingdao 266109,China; 4.Dept.of Chemistry,Hanshan Normal College,Chaozhou 521041,China) |
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| Abstract: | The method of microchip electrophoresis with laser induced fluorescence detection was proposed to be used in the separation and determination of catecholamines,including norepinephrine(NE),dopamine(DA) and epinephrine(E).The application of a self-made pump-free negative pressure sample introduction system made it possible to avoid the discrimination effect in sample introduction.NE,DA and E were separated completely within 1 min under the optimum working conditions,with their average migration times of 30.59,37.23,46.43 s and values of RSD′s(n=7) of 1.10%,1.28%,0.45% respectively.Linearity ranges found were 0.3-5.0 mg·L-1(for NE and DA) and 0.05-4.0 mg·L-1(for E),with detection limits(3S/N) of 30,30,10 μg·L-1 respectively. |
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| Keywords: | Microchip electrophoresis Laser induced fluorescence spectrometry Catecholamines |
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