A Simple Method to Measure Plasma Levels of Propafenone with Fluorescence Detection |
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| Authors: | C Flores-Pérez H Juárez-Olguín J Flores-Pérez B Ramírez-Mendiola J Bobadilla Chávez |
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| Institution: | (1) Laboratorio de Farmacología, Instituto Nacional de Pediatría (INP), Avenida Imán No.1 3er piso, Colonia Cuicuilco, CP 04530 México D.F., México;(2) Departamento de Farmacología, Facultad de Medicina, Universidad Nacional Autónoma de México, México;(3) Servicio de Cardiología, INP, México D.F., México |
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| Abstract: | The aim of the present study was to develop a simple method to measure plasma levels of propafenone by liquid chromatography
with a C18 reverse-phase column and fluorescence detection, without previous derivatization of the sample. Linearity was assessed
in the range from 50 to 1000 ng mL−1 and had a correlation coefficient of 0.999. The inter- and intra-day coefficients of variation were below 5%. The limits
of detection and quantification were 15 ng mL−1 and 50 ng mL−1, respectively. Drug levels were determined satisfactorily in two patients. A simple and reliable method was developed, especially
useful in children with heart failure under treatment with propafenone. |
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| Keywords: | Column liquid chromatography Propafenone Plasma levels Pharmacokinetics |
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