微量量热法研究螺旋藻活性物质对癌细胞和正常细胞的作用

螺旋藻对人体生理的作用已引起人们的重视,特别是在增强免疫力和抗癌方面。本文用标准型(MS 80)Calvet微量量热计测定了正常生长状况下人宫颈癌传代细胞(Hela)、人乳腺癌细胞(Bcap-37)和正常人胚肺二倍体细胞(2BS)的增殖热谱图;螺旋藻活性物质(Sp,P)对上述癌细胞和正常细胞作用的热谱图。同时,测定了量热实验前后的细胞数、Sp.S对细胞生长的抑制率和细胞死亡率。

Stady of the Effect on the Growth of Tumor and Normal Cells with Bioactive Materials of Spirulina by Microcalorimetry Method

The Ms 80 standard calvet microcalorimeter was used for measuring the thermo-grams of Hela, human breast carcinoma(Bcap-37) and diploid fibroblasts from human fetal lung (2BS) at 37 ℃ under the conditions of normal cell culture and culture in the presence of bioactive materials in Spirulina. There is a difference in the shapes of thermograms beteween tumor cells (Hela, Bcap-37) and normal coll (2BS) under the same condition. The former tended to ascent and the latter was almost parallel to abscissa during the measurements. The cell number was counted and the inhibit rate of growth and the death rate were obtained. The Sp. P was growth inhibitor and lethal to tumo cells, but had no influence on the growth of 2BS at the concentration of 100 μg·ml~(-1).