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Measurement of Homonuclear J-Couplings from Spin-State Selective Double-/Zero-Quantum Two-Dimensional NMR Spectra |
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| Authors: | Perttu Permi Sami Heikkinen Ilkka Kilpelinen Arto Annila |
| Institution: | a Institute of Biotechnology, University of Helsinki, P.O. Box 56, FIN-00014, Helsinki, Finland;b VTT Chemical Technology, P.O. Box 1401, FIN-02044 VTT, Espoo, Finland |
| Abstract: | 1H-detected two-dimensional double-/zero-quantum experiments are described for measurement of homonuclear 2JHH-couplings of NH2 or CH2 groups in proteins. These experiments utilize multiple-quantum coherence for determination of the size and the absolute sign of the geminal scalar and dipolar couplings in the presence of broad lines. Spectra are simplified by gradient selection and spin-state selective filters. |
| Keywords: | couplings HB-GAM multiple-quantum coherence spin-state-selective filters ubiquitin |
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