Electrokinetic DNA transport in 20 nm-high nanoslits: evidence for movement through a wall-adsorbed |
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| Authors: | Castillo-Fernandez Oscar Salieb-Beugelaar Georgette B van Nieuwkasteele Jan W Bomer Johan G Arundell Martin Samitier Josep van den Berg Albert Eijkel Jan C T |
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| Affiliation: | Nanobioengineering Group, Institute for Bioengineering of Catalonia, Josep Samitier, Barcelona, Spain. |
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| Abstract: | The electrokinetic transport behavior of λ-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation. |
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