|
|||||
|
|
Comparison of UPLC and HPLC for Analysis of Dietary Folates |
|
| Authors: | Johan Jastrebova Hanna S Strandler Jelena Patring Tony Wiklund |
| Institution: | 1. Department of Food Science, Swedish University of Agricultural Sciences, Box 7051, 750 07, Uppsala, Sweden 2. The National Food Administration, Box 622, 751 26, Uppsala, Sweden 3. Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences, Box 7050, 750 07, Uppsala, Sweden 4. Waters Sweden, Box 485, 191 24, Sollentuna, Sweden |
| Abstract: | Ultra performance liquid chromatography (UPLC) using small sub-2 ??m particles and high performance liquid chromatography (HPLC) were compared for separation and determination of the most common dietary folates; 5-methyltetrahydrofolate, tetrahydrofolate, 5-formyltetrahydrofolate, 10-formylfolic acid and folic acid. Two UPLC columns??Acquity BEH C18 and Acquity HSS T3, and two HPLC columns with similar surface chemistry??Xbridge C18 and Atlantis d18 were tested. When using UPLC, the signal-to-noise ratio could be improved by a factor of 2?C50 for different folate derivatives and the run time could be reduced fourfold without sacrificing separation efficiency. The applicability of UPLC to real food samples was demonstrated. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |